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Comparison of four extraction methods to detect hepatitis A virus RNA in serum and stool samples BJID
Paula,Vanessa Salete de; Villar,Livia Melo; Gaspar,Ana Maria Coimbra.
The efficiency of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as Brazil. We compared the efficiency of four different extraction techniques in serum and stool samples for the detection of hepatitis A virus by reverse transcription PCR (RT-PCR). We used PCR to analyse serum and stool samples of 12 patients who were referred to the Brazilian Reference Center for Viral Hepatitis (BRCVH) in Rio de Janeiro. The methods tested were Proteinase K, Silica, TRIzol and Guanidine isothiocyanate. Proteinase K extraction was the best method for serum samples; it detected the HAV-RNA in 11 of the 12 samples. The guanidine isothiocyanate method was the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HAV; Extraction methods; Serum and stool; RT-PCR.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200007
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Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil BJID
Santos,Damião Carlos Moraes dos; Martinho,José Manoel da Silva Gomes; Pacheco-Moreira,Lucio Filgueiras; Araújo,Cristina Carvalho Viana de; Oliveira,Barbara Cristina Euzebio Pereira Dias de; Lago,Barbara Vieira; Pinto,Marcelo Alves; Paula,Vanessa Salete de.
Fulminant hepatic failure (FHF) is characterized by massive hepatocellular injury, whose physiopathology is still unclear. Hepatitis B (HBV) is probably the most common viral cause of FHF, while hepatitis A (HAV) virus seem occurs less frequently. However, the host and viral factors that determine the outcome of these infections are poorly understood. In the present study, viral load and genotyping determining regions of HAV and HBV genomes were sequenced. Eight FHF patients and one patient with severe acute hepatitis (SAH) were included. Liver and blood samples were collected during liver transplantation or necropsy procedures. HAV-RNA and HBV-DNA were extracted from serum, biopsy and paraffin liver. Nucleotide sequencing of HAV-RNA was performed from...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Fulminant hepatitis failure; Hepatitis A virus (HAV); Hepatitis B virus (HBV) and genotype and viral load.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000500002
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RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions BJID
Silva,Amanda Perse da; Lopes,Juliana Freitas; Paula,Vanessa Salete de.
The aim of this study was to evaluate the use of RNA interference to inhibit herpes simplex virus type-1 replication in vitro. For herpes simplex virus type-1 gene silencing, three different small interfering RNAs (siRNAs) targeting the herpes simplex virus type-1 UL39 gene (sequence si-UL 39-1, si-UL 39-2, and si-UL 39-3) were used, which encode the large subunit of ribonucleotide reductase, an essential enzyme for DNA synthesis. Herpes simplex virus type-1 was isolated from saliva samples and mucocutaneous lesions from infected patients. All mucocutaneous lesions' samples were positive for herpes simplex virus type-1 by real-time PCR and by virus isolation; all herpes simplex virus type-1 from saliva samples were positive by real-time PCR and 50% were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Herpes simplex virus type 1; Real-time PCR; SiRNA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400441
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